Experimental data & uncertainty · SL/HL
Biology Calibration Curve Calculator
Enter known standards and responses, then the response of the unknown.
Your values
Start with the example or enter your own values. Use a dot for decimals and e for scientific notation.
Calculated locally. No account or input upload.
Result & working
ExampleThe calculator is loading. The worked example is also shown below.
Step-by-step working
The formula
OLS fit: y = mx + c
unknown x = (y_unknown − c)/m
| Symbol | Meaning |
|---|---|
| R² | Fraction of response variation described by the fitted linear trend. |
When to use this calculator
Common in colorimetry and other biological assays. The unknown is most defensible within the calibrated range.
Worked example
The page opens with these example values. The result below is pre-generated for reference.
- OLS fit: y = 0.199x + 0.008
- R² = 0.9987138
- x_unknown = (y_unknown − c)/m = (0.5 − (0.008))/0.199 = 2.472362 mg cm⁻³
2.472362 mg cm⁻³
Common mistakes
- Do not assume a high R² proves the method has no systematic bias.